Skills and Certifications

Programming Skills

R, Python, Linux, Bash scripting, High Performance Computing (SLURM), Git, Pipeline management (Snakemake, Nextflow), Code documentation, MATLAB, and SQL

Bioinformatics

NGS Data Analysis, Bulk and Single Cell RNA-seq, TCR-seq, CITE-seq, Spatial Transcriptomics; Whole Genome Sequencing, Metagenomics, Cancer Genomics, Variant Calling, Homology Identification, Multiple Sequence Alignment, and Phylogeny; ChIP-seq, CUT&Tag; Long-read Sequence Assembly

Key tools:

• Bulk RNA-seq: DESeq2, Limma, edgeR, msigdbr, clusterProfiler, variancePartition, maSigPro, WGCNA
• Single Cell RNA-seq: Seurat, Harmony, scCustomize, scRepertoire, Monocle
  • Cellltype Annotation: Azimuth, singleR, celldex, Label Transfer, ProjecTILs for CD cells
• Whole Genome Sequencing: GATK, kraken2, centrifuger, Rsamtools, karyoploteR
• Methylation projects: Diffbind3, Deseq2, UROPA
• Data Visualization and Reporting: DT, htmltools, KnitR, PCAtools, plotly, EnrichPlot, EnhancedVolcano, ComplexHeatmap, tidyverse

Molecular Biology

Cell culture, RNA extraction, Protein engineering, HPLC, FPLC, Cell-free systems, Cloning, and transformation

Professional Development

• HMX Fundamentals: Immunology, Harvard Medical School (2024)
• HMX Fundamentals: Genetics, Harvard Medical School (2024)
• Mayo Illinois Computational Genomics Course, University of Illinois and Mayo Clinic (2021)
• Executive General Management Program, IIM Bangalore, India (2013)